cDNA Encoding Tropinone Reductase-II from Hyoscyamus niger
نویسندگان
چکیده
منابع مشابه
cDNA encoding tropinone reductase-II from Hyoscyamus niger.
Two stereospecific NADPH-dependent reductases, TR-I and TR-11, constitute a branching point in the biosynthesis of tropane alkaloids. TR-I catalyzes the stereospecific reduction of tropinone to tropine (Koelen and Gross, 1982), whereas TR-I1 reduces tropinone to pseudotropine (Drager et al., 1988). We previously characterized TRs that had been purified from cultured roots of Hyoscyamus niger (H...
متن کاملStructures and expression patterns of two tropinone reductase genes from Hyoscyamus niger.
In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same e...
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Tropinone is an alkamine intermediate at the branch point of biosynthetic pathways leading to various tropane alkaloids. Two stereospecifically distinct NADPH-dependent oxidoreductases, TR-I and TR-II, which, respectively, reduce tropinone to 3alpha-hydroxytropane (tropine) and 3beta-hydroxytropane (psi-tropine), were detected mainly in the root of tropane alkaloid-producing plants but not in n...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1993
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.103.4.1465